Composite

Part:BBa_K4728020

Designed by: Bryant Han   Group: iGEM23_Queens-Canada   (2023-10-10)


TPA transporter expression (TpaK)

Constitutive expression of the TPA transporter proteins (TpaK) and the TphR, the TPA-binding activator. This part is meant to be used with K4728017, which expresses eiraCFP under a TPA-inducible promoter. TpaK comes from the genome of Rhodococcus jostii and TphR comes from the genome of Comamonas testosteroni.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1878
    Illegal PstI site found at 1984
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1878
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1984
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1878
    Illegal BglII site found at 640
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1878
    Illegal PstI site found at 1984
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1878
    Illegal PstI site found at 1984
    Illegal AgeI site found at 412
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None